For each species, select a minimum of 30 focal trees per treatment. Treatments should be chosen based on their relevance to the monitoring objectives. For instance, trees that have undergone prescribed burning, control/unburned trees, trees exposed to different levels of fire severity (e.g., those with 30% crown scorch), among others. The inclusion of unburned/control trees is strongly recommended for comparison purposes.
To ensure a valid comparison between treatment groups, select trees with similar initial DBH, unless DBH itself is the focus of a treatment group. In such cases, ensure that trees within each group are comparable in size. This approach helps isolate the effects of burning from any size-related growth differences.
Focal trees should be spaced as far apart as possible within the plot to minimize spatial autocorrelation. Each tree must be clearly labeled, and its coordinates recorded using a sub-metric GPS system to facilitate future surveys.